Determination of functional domains in the C subunit of the CCAAT-binding factor (CBF) necessary for formation of a CBF-DNA complex: CBF-B interacts simultaneously with both the CBF-A and CBF-C subunits to form a heterotrimeric CBF molecule
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چکیده
منابع مشابه
Three classes of mutations in the A subunit of the CCAAT-binding factor CBF delineate functional domains involved in the three-step assembly of the CBF-DNA complex.
The mammalian CCAAT-binding factor CBF (also called NF-Y or CP1) consists of three subunits, CBF-A, CBF-B, and CBF-C, all of which are required for DNA binding and present in the CBF-DNA complex. In this study we first established the stoichiometries of the CBF subunits, both in the CBF molecule and in the CBF-DNA complex, and showed that one molecule of each subunit is present in the complex. ...
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To understand the role of the CCAAT-binding factor, CBF, in transcription, we developed a strategy to purify the heterotrimeric CBF complex from HeLa cell extracts using two successive immunoaffinity chromatography steps. Here we show that the p32 protein, previously identified as the ASF/SF2 splicing factor-associated protein, copurified with the CBF complex. Studies of protein-protein interac...
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Neurovascular coupling associated with visual and vibrotactile stimulations in baboons anesthetized sequentially with isoflurane and ketamine was evaluated using multimodal functional magnetic resonance imaging (fMRI) on a clinical 3-Tesla scanner. Basal cerebral blood flow (CBF), and combined blood-oxygenation-level-dependent (BOLD) and CBF fMRI of visual and somatosensory stimulations were me...
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A scintillation camera connected to a dedicated computer system was employed for atraumatic CBF measurements in 43 patients after intravenous injection of 35 to 55 raCi Xe in saline solution. To validate this method results were compared to flow values in the same patients measured after intracarotid Xe injection. While the correlation between i.e. and i.v. values was not satisfactory when a hi...
متن کاملThe Lower Limit of Cbf Autoregulation
Copyright © 2013 International Anesthesia Research Society PERSPECTIVE I have committed considerable professional time to anesthesia for neurologic surgery. That commitment has caused me to hear a great deal related to neuroanesthesia. I have been asked by faculty colleagues in anesthesia, “What do you suppose might have gone wrong during that case yesterday?” I have listened to American Board ...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1996
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.16.8.4003